anti-Il12b antibody product blog
Tags: Antibody; IL-12; Polyclonal Antibody; anti-IL12B antibody; IL12B;
The Il12b il12b (Catalog #MBS220122) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s IL-12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).ELISA: This biotinylated mouse IL-12 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified mouse IL-12 antibody as the capture reagent and recombinant mouse IL-12 as the standard.
Western Blot: This biotinylated mouse IL-12 antibody may used in Western Blotting application under either reducing of non-reducing conditions with recombinant mouse IL-12 as the positive control.
ELISA: Minimum Dilution: 0.25; Maximum Dilution: 1.0ug/ml
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml. Researchers should empirically determine the suitability of the Il12b il12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Il12b il12b product has the following accession number(s) (GI #6680397) (NCBI Accession #NP_032378.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Goat anti Mouse Interleukin-12 antibody recognizes mouse interleukin-12 (IL-12). Interleukin-12 is produced by activated B lymphocytes and macrophages. The properties of IL-12 include the stimulation of growth of activated T lymphocytes and the development of TH1 CD4 +ve cells and CD8 +ve cytotoxic T cells. Goat anti Mouse Interleukin-12 antibody neutralizes murine IL-12 activity with an ND50 for mIL-12 (2ngml-1 of between 0.002 and 0.002μg ml-1.
Reconstitution: Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. Immunogen: Recombinant mouse IL-12 (PMP40)
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to mouse IL-12 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Il12b are readily searchable from our website. Different antibodies against the same target such as Il12b may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.