anti-IL-1 antibody product blog
Tags: Antibody; IL-1; Monoclonal Antibody; IL-1 beta; anti-IL-1 antibody;
The IL-1 il1b (Catalog #MBS550073) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Mouse anti Human IL-1 beta reacts with Human and may cross-react with other species as described in the data sheet.Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of IL-1b (10 ug/ml), a concentration of 4-8 ug/ml of this antibody is required.
ELISA:This antibody can be used at 1 ug/mL with the appropriate secondary reagents to detect human VEGF. Researchers should empirically determine the suitability of the IL-1 il1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IL-1 il1b product has the following accession number(s) (GI #10835145) (NCBI Accession #NP_000567.1) (Uniprot Accession #P01584). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Monocytes are the main source of secreted IL1. They express predominantly IL1- beta while human keratinocytes express large amounts of IL1-alpha. Murine macrophages display a transition from IL1-beta to IL1-alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL1, IL1-alpha and IL1-beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL1-beta but not the IL1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.
Host Note: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human IL-1 beta. Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IL-1 are readily searchable from our website. Different antibodies against the same target such as IL-1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.