anti-IgG (H+L) (human absorbed) antibody product blog
Tags: Antibody; Polyclonal Antibody; IgG (H+L) (human absorbed); anti-IgG (H+L) (human absorbed) antibody;
The IgG (H+L) (human absorbed) n/a (Catalog #MBS524015) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse IgG (H+L) (human absorbed), FITC, (Polyclonal) (goat IgG) reacts with Mouse and may cross-react with other species as described in the data sheet.This antibody is tested by flow cytometry. From this testing, we recommend using between 0.1 and 0.25 ug of antibody for 1x10^6 cells in a 100 ul staining volume. Lesser amounts of reagent may be sufficient and it is recommended that the customer determine the optimum amount for each application. Researchers should empirically determine the suitability of the IgG (H+L) (human absorbed) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
FITC Goat anti-Mouse IgG (H+L), human adsorbed.
Preparation: Antibodies were affinity purified by column chromatography and adsorbed to remove cross reactivity to human immunoglobulins. The resulting antibody was conjugated with FITC to provide the optimal fluorochrome to protein ratio for flow cytometry. Any free dye is removed by column chromatography
Buffers and Preservatives: This reagent is provided in 10 mM Sodium phosphate, 0.15 M Sodium chloride, and it contains 0.05% sodium azide as a preservative. A highly purified grade of BSA has been added as a stabilizing protein to bring the final protein concentration to 4-5 mg/ml after conjugation. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IgG (H+L) (human absorbed) are readily searchable from our website. Different antibodies against the same target such as IgG (H+L) (human absorbed) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.