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anti-IgG antibody product blog

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Posted on 2014-08-02 05:56:47 by mybiosource_staff
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Tags: Antibody; IgG; Polyclonal Antibody; anti-IgG antibody;
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The IgG n/a (Catalog #MBS235194) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s IgG can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Western Blot (WB).
Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/100;
ELISA: Minimum Dilution: 1/6400; Maximum Dilution: 1/12800;
Western Blotting: Maximum Dilution: 1/10000. Researchers should empirically determine the suitability of the IgG n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The IgG n/a product has the following accession number(s) (GI #121088) (NCBI Accession #P01870.1) (Uniprot Accession #P01870). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SHEEP ANTI RABBIT IgG:HRP (MULTI ADSORBED) with the following immunoassay(s):
Testing Data #1 (Detection of Rabbit anti Human MAP1LC3A/B (MBS224211) in a Western blot of HeLa cell lysate using Sheep anti Rabbit IgG:HRP (MBS235194))
Testing Data #1 IgG.

Testing Data #2 (Detection of Rabbit anti Human MAML3 (MBS224278) in an immunohistochemical analysis of formalin fixed paraffin embedded mouse intestine using Sheep anti Rabbit IgG:HRP (MBS235194))
Testing Data #2 IgG.

Testing Data #3 (Detection of Rabbit anti Human XRCC4 (MBS222384) in a Western blot analysis of a HeLa cell nuclear extract using Sheep anti Rabbit IgG:HRP (MBS235194))
Testing Data #3 IgG.

Testing Data #4 (Detection of Rabbit anti Human SP1 (MBS221750) in a Western blot analysis of a HeLa cell nuclear extract using Sheep anti Rabbit IgG:HRP (MBS235194))
Testing Data #4 IgG.

MBS235194 recognises rabbit IgG as demonstrated by ELISA and double diffusion. Cross-reactivity against human, mouse, rat and bovine immunoglobulins is reduced by solid phase adsorption.

Perservative Stabilisers: 0.05% ProclinTM300
0.002% Methylisothiazolone
0.002% Bromonitrodioxane. Immunogen: Purified rabbit IgG from normal rabbit serum
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species: Rabbit. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IgG are readily searchable from our website. Different antibodies against the same target such as IgG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.
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