anti-IGF1R antibody product blog
Tags: Antibody; anti-IGF1R antibody; IGF1R; Monoclonal Antibody; Insulin-Like Growth Factor I Receptor, beta;
The IGF1R igf1r (Catalog #MBS632908) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Insulin-Like Growth Factor I Receptor, beta, phosphorylated (Tyr1135) (IGF1Rb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Insulin-Like Growth Factor I Receptor, beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Researchers should empirically determine the suitability of the IGF1R igf1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IGF1R igf1r product has the following accession number(s) (GI #4557665) (NCBI Accession #NP_000866.1) (Uniprot Accession #P08069). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Insulin-Like Growth Factor I Receptor, beta, phosphorylated (Tyr1135) (IGF1Rb) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from MCF-7 cells, untreated or stimulated with IGF-I, using Phospho-IGF-I Receptor (Tyr1135) mAb (upper) and MBS632908 (lower).)
Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphoryla-tion follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) are the earliest major autophosphory-lation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and func-tional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine auto-phosphorylation of the insulin receptor is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr1135 of human IGF-I receptor Beta (KLH-coupled). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IGF1R are readily searchable from our website. Different antibodies against the same target such as IGF1R may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.