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anti-IFN BETA antibody product blog

Posted on 2015-06-15 10:00:46 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; IFN BETA; anti-IFN BETA antibody;
The IFN BETA ifnb1 (Catalog #MBS223102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI IFN BETA reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s IFN BETA can be used in a range of immunoassay formats including, but not limited to, Immunohistology Paraffin*, Western Blot (WB).
Western Blot: This item detects a band of approximately 15kDa in A20 cell lysates.
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Maximum Dilution: 5ug/ml. Researchers should empirically determine the suitability of the IFN BETA ifnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The IFN BETA ifnb1 product has the following accession number(s) (GI #4504603) (NCBI Accession #NP_002167.1) (Uniprot Accession #P01574). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI IFN BETA with the following immunoassay(s):
Testing Data #1 (Western blot analysis of whole cell lysate from A-20 mouse B-cell lymphoma probed with Rabbit anti Human IFN beta (MBS223102) )
Testing Data #1 IFN BETA.

Testing Data #2 (Formalin fixed, paraffin embedded human liver stained with Rabbit anti Human IFN beta (MBS223102) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Testing Data #2 IFN BETA.

MBS223102 recognises interferon beta (IFN-beta), a 22kD member of the type I alpha/beta interferon family. IFN beta, initially described as fibroblast interferon, is produced predominantly by fibroblasts and some epithelial cells. Type 1 interferons are produced primarily in response to viral infections as part of the host immune response and can also inhibit the development of tumours. IFN-beta is involved in the regulation of non-specific humoural immune responses and responses against viral infections.

Perservative Stabilisers: 0.02% Sodium Azide. Immunogen: A 17 amino acid peptide located near the centre of human IFN Beta.
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to human IFN Beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IFN BETA are readily searchable from our website. Different antibodies against the same target such as IFN BETA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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