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anti-IFIH1 antibody product blog

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Posted on 2015-03-23 04:57:30 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; MDA5; anti-IFIH1 antibody; IFIH1;
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The IFIH1 ifih1 (Catalog #MBS150964) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MDA5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s MDA5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the IFIH1 ifih1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The IFIH1 ifih1 product has the following accession number(s) (GI #27886568) (NCBI Accession #NP_071451) (Uniprot Accession #Q9BYX4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MDA5 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.)
Western Blot (WB) IFIH1.

Immunohistochemistry (IHC) (Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 μg/mL.)
Immunohistochemistry (IHC) IFIH1.

Immunofluorescence (IF) (Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.)
Immunofluorescence (IF) IFIH1.

MDA5 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-κB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.

Conjugate: Unconjugated
Immunogen: MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5.
Buffer: MDA5 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IFIH1 are readily searchable from our website. Different antibodies against the same target such as IFIH1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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