anti-ICDH antibody product blog
Tags: Antibody; Polyclonal Antibody; Isocitrate Dehydrogenase; anti-ICDH antibody; ICDH;
The ICDH f20d21.16 (Catalog #MBS624120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Isocitrate Dehydrogenase, CT (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial [Precursor], Isocitric dehydrogenase, NAD(+)-specific ICDH, IDH3A, IDH3) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Isocitrate Dehydrogenase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the ICDH f20d21.16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ICDH f20d21.16 product has the following accession number(s) (GI #15221788) (NCBI Accession #NP_175836.1) (Uniprot Accession #Q9SLK0). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Isocitrate Dehydrogenase, CT (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial [Precursor], Isocitric dehydrogenase, NAD(+)-specific ICDH, IDH3A, IDH3) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Isocitrate dehydrogenase in MCF-7, Jurkat cell line lysates (35ug/lane). Isocitrate dehydrogenase (arrow) was detected using the purified Pab.)
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
Immunogen: Synthetic peptide selected from the C-terminal region of human Isocitrate dehydrogenase (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ICDH are readily searchable from our website. Different antibodies against the same target such as ICDH may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.