anti-HSPA1A antibody product blog
Tags: Antibody; HSP70; Polyclonal Antibody; HSPA1A; anti-HSPA1A antibody;
The HSPA1A hspa1a (Catalog #MBS176327) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hsp70 antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Hsp70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC.Western Blot
Concentration: 0.1-0.5 ug/mL
Tested Species: Hu
Immunohistochemistry (Paraffin-embedded Section)
Concentration: 0.5-1 ug/mL
Tested Species: Hu
Antigen Retrieval: By Heat
Immunohistochemistry (Frozen Section)
Concentration: 0.5-1 ug/mL
Tested Species: Rat
Predicted Species: Hu
Immunohistochemistry
Concentration: 0.5-1 ug/mL
Tested Species: Hu
Tested Species: In- house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users. . Researchers should empirically determine the suitability of the HSPA1A hspa1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSPA1A hspa1a product has the following accession number(s) (GI #147744565) (NCBI Accession #P08107.5) (Uniprot Accession #P08107). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Hsp70 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-Hsp70 antibody, Western blotting
All lanes: Anti Hsp70 at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Predicted bind size: 70KD
Observed bind size: 70KD)
Immunohistochemistry (IHC) (Anti-Hsp70 antibody, PA1813, IHC(P)
IHC(P): Human Intestinal Cancer Tissue)
Immunocytochemistry (ICC) (Anti-Hsp70 antibody, ICC
ICC: A549 Cell)
Immunohistochemistry (IHC) (Anti-Hsp70 antibody, PA1813, IHC(F)
IHC(F):Rat Intestine Tissue)
Description: Rabbit IgG polyclonal antibody for Heat shock 70 kDa protein 1A/1B(HSPA1A) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Rat.
Background: HSPA1(heat shock 70kDa protein 1A)�also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a�protein�that in humans is encoded by the�HSPA1A�gene. This�intronless�gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family.�The HSPA1A gene encodes a predicted 641-amino acid protein.�The HSPA1 gene is mapped on 6p21.33. Shimizu et al.(1999)�found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.