anti-HSP90B1 antibody product blog
Tags: Antibody; Polyclonal Antibody; HSP90B1; anti-HSP90B1 antibody; Glucose Regulated Protein 94;
The HSP90B1 hsp90b1 (Catalog #MBS621136) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glucose Regulated Protein 94 (Grp94, gp96) reacts with Canine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Glucose Regulated Protein 94 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC).Suitable for use in Western Blot, Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:80
Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the HSP90B1 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSP90B1 hsp90b1 product has the following accession number(s) (GI #553324) (Uniprot Accession #P14625). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Glucose Regulated Protein 94 (Grp94, gp96) with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis of human small intestine tissue stained with MBS621136 at 10ug/ml.)
Glucose-regulated protein 94, also known as Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which belongs to the Hsp90 family of molecular chaperones. Grp94 possesses the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL), a sorting signal thought to lead to the retention of proteins in the pre-Golgi compartments (1). Grp94 expression is upregulated by stress conditions which promote protein misfolding or unfolding (2). In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides (3,4). Grp94 and Hsp90 share high sequence identity and presumably identical adenosine nucleotide-dependent modes of regulation. Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the binding site for the Hsp90 inhibitors geldanamycin and radicicol. Recent data has identified a ligand dependent regulation of Grp94 function and suggest a model whereby Grp94 function is regulated through a ligand-dependent conversion of Grp94 from an inactive to an active conformation (5, 6).
Immunogen: A synthetic peptide derived from sequence near the carboxy-terminus of mouse Grp94. Positive Control: Mouse Brain Tissue Extract or Rat Brain Tissue Extract. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HSP90B1 are readily searchable from our website. Different antibodies against the same target such as HSP90B1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.