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anti-HSP90B1 antibody product blog

Posted on 2015-01-14 08:27:14 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; HSP90B1; anti-HSP90B1 antibody; GPR94/TRA1;
The HSP90B1 hsp90b1 (Catalog #MBS420298) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GPR94/TRA1 Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish and may cross-react with other species as described in the data sheet. MyBioSource\'s GPR94/TRA1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Liver shows staining in some hepatocyte nuclei and weak cytoplasm staining of hepatocytes. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 100kDa band observed in lysates of Mouse and Rat Liver and of cell lines NIH3T3 and NSO (calculated MW of 92.5kDa according to NP_003290.1). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the HSP90B1 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The HSP90B1 hsp90b1 product has the following accession number(s) (GI #4507677) (NCBI Accession #NP_003290.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-GPR94/TRA1 Antibody with the following immunoassay(s):
Western Blot (WB) ((0. 03ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) HSP90B1.

Immunohistochemistry (IHC) ((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC) HSP90B1.

Immunogen: Peptide with sequence C-KEGVKFDESEKTKE, from the internal region of the protein sequence according to NP_003290.1.
Epitope: Internal region. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HSP90B1 are readily searchable from our website. Different antibodies against the same target such as HSP90B1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Bone, Bone Marrow, Brain, Liver, Lung, Lymph Node, Nerve, Prostate, Testis tissues are correlated with this protein. Breast Neoplasms, Carcinoma, Hepatocellular, Disease Models, Animal, Hyperplasia, Inflammation, Liver Diseases, Liver Neoplasms, Lung Diseases, Nervous System Diseases, Testicular Diseases are some of the diseases may be linked to Goat anti-GPR94/TRA1 Antibody.
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