|The HSP20 n/a (Catalog #MBS531283) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSP20 antibody reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s HSP20 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the HSP20 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSP20 n/a product has the following accession number(s) (GI #15841518) (NCBI Accession #NP_336555.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HSP20 antibody with the following immunoassay(s):
ELISA (ELISA analysis using Hsp20 antibody
12-weeks old mice (FVB/N) underwent in vivo 30-min myocardial ischemia, via coronary artery occlusion, followed by 24-h reperfusion. Subsequently, sera were collected and levels of Hsp20 were measured using Hsp20 as the detection antibody in an optimized Sandwich ELISA. Image taken from Zhang et al, Hsp20 functions as a novel cardiokine in promoting angiogenesis via activation of VEGFR2; Pubmed ID: 22427880 (see Reference Tab))
Immunofluorescence (Immunofluorescent staining using Hsp20 antibody
Hsp20 was used to examine recombinant human Hsp20 protein in Hsp20-transgenic mice section and co-localized with VEGFR2 in the cell surface (green, Hsp20; red, VEGFR2). Image taken from Zhang et al, Hsp20 functions as a novel cardiokine in promoting angiogenesis via activation of VEGFR2; Pubmed ID: 22427880 (see Reference Tab))
Western Blot (Western Blot analysis using Hsp20 antibody
Protein samples were extracted from Hsp20-transgenic mice or cultured HUVECs. Binding of Hsp20 antibody (1:500) was detected by peroxidase-conjugated secondary antibodies and enhanced chemiluminescence. Alpha Actin was used as an internal control. Image taken from Zhang et al, Hsp20 functions as a novel cardiokine in promoting angiogenesis via activation of VEGFR2; Pubmed ID: 22427880 (see Reference Tab))
Mouse monoclonal HSP20 antibody.
Biological Significance: Heat shock proteins (HSP) are a class of functionally related proteins whose expression is increased when cells are exposed to elevated temperatures or other stress. This increase in expression is transcriptionally regulated. HSPs are found in virtually all living organisms, from bacteria to humans and are named according to their molecular weight. Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen: HSP20 antibody was raised in mouse using recombinant human HSP20 as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HSP20 are readily searchable from our website. Different antibodies against the same target such as HSP20 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.