anti-HRG antibody product blog
Tags: Antibody; Polyclonal Antibody; Histidine-rich Glycoprotein; anti-HRG antibody; HRG;
The HRG hrg (Catalog #MBS625795) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histidine-rich Glycoprotein, NT (HRG, HRGP, Histidine-proline-rich Glycoprotein, HPRG) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Histidine-rich Glycoprotein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the HRG hrg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HRG hrg product has the following accession number(s) (GI #2280514) (Uniprot Accession #P04196). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Histidine-rich Glycoprotein, NT (HRG, HRGP, Histidine-proline-rich Glycoprotein, HPRG) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of HRG antibody (N-term) in K562 cell line lysates (35ug/lane). HRG (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (HRG Antibody (N-term) (RB18729) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HRG Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
HRG contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein\'s effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels.
Immunogen: Synthetic peptide selected from the N-terminal region of human HRG (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HRG are readily searchable from our website. Different antibodies against the same target such as HRG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.