anti-HK2 antibody product blog
Tags: Antibody; HK2; Monoclonal Antibody; anti-HK2 antibody; Hexokinase 1;
The HK2 hk2 (Catalog #MBS200075) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hexokinase 1, 2 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Hexokinase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF).The antibody recognizes all four isoforms of Hexokinase (1, 2) in recombinant protein.WB: 1:1,000 ~ 3,000.
Recommended starting dilution is 1:2,000. Researchers should empirically determine the suitability of the HK2 hk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HK2 hk2 product has the following accession number(s) (GI #15553127) (NCBI Accession #NP_000180) (Uniprot Accession #P52789). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hexokinase 1, 2 antibody with the following immunoassay(s):
Western Blot (WB) (The Recombinant Proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase I, II antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Hexokinase 1 recombinant protein Lane 2.: Hexokinase 2 recombinant protein Lane 3.: Hexokinase 3 recombinant protein Lane 4.: Hexokinase 4 recombinant protein The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase I, II antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: K562 cell lysate Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection)
Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.
Antigen Species: Human
Immunogen: Recombinant human Hexokinase 2 (1-917aa) purified from E. coli. Clone Note: Anti-human Hexokinase 2 mAb, clone 1A7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hexokinase 2 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HK2 are readily searchable from our website. Different antibodies against the same target such as HK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.