anti-HISTIDINE TAG antibody product blog
Tags: Antibody; Polyclonal Antibody; HISTIDINE TAG; anti-HISTIDINE TAG antibody;
The HISTIDINE TAG n/a (Catalog #MBS223951) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s HISTIDINE TAG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)*, Western Blot (WB).ELISA: Minimum Dilution: 1/1000; Maximum Dilution: 1/30000; Application Note: We recommend a dilution range of 1/1000 to 1/30000 for use in indirect ELISA. When coating the antibody directly on to a plate, MyBioSource recommend a dilution range to 1/100 to 1/500. Researchers should empirically determine the suitability of the HISTIDINE TAG n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Goat anti-Histidine tag antibody recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Goat anti-Histidine tag antibody may not detect all N-Terminal (NT) histidine tagged proteins. Goat anti-Histidine tag antibody has been shown to be suitable for use on cytospins.
Perservative Stabilisers: 0.1% Sodium Azide (NaN3). Immunogen: Keyhole limpet haemocyanin (KLH) conjugated H-H-H-H-H-H
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antiserum to Histidine Tag was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species: Synthetic Peptide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HISTIDINE TAG are readily searchable from our website. Different antibodies against the same target such as HISTIDINE TAG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.