anti-HAVCR1 antibody product blog
Tags: Antibody; Polyclonal Antibody; TIM-1; anti-HAVCR1 antibody; HAVCR1;
The HAVCR1 havcr1 (Catalog #MBS150460) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIM-1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s TIM-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).TIM-1 antibody can be used for the detection of TIM-1 by Western blot at 1 - 2 mug/mL. Knockdown (Knockout, KO validated) Antibody. Researchers should empirically determine the suitability of the HAVCR1 havcr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HAVCR1 havcr1 product has the following accession number(s) (GI #153085427) (NCBI Accession #NP_036338) (Uniprot Accession #Q96D42). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TIM-1 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1 Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: TIM-1 3811 (1 μg /mL), 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
)
Western Blot (WB) (Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: TIM-1 3809 (8 μg /mL), TIM-1 3811 (1 μg/mL), ?-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
)
Western Blot (WB) (Figure 3 Validation with TIM-1 siRNA Knockdown
HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2)
Loading: 15 μg of HeLa whole cell lysates per lane.
Antibodies: 3811 (1 μg /mL), 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
)
TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.
Conjugate: Unconjugated
Immunogen: TIM-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-1.
Buffer: TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.