anti-H3K4me3 antibody product blog
Tags: Antibody; H3K4me3; anti-H3K4me3 antibody;
The H3K4me3 hist3h3 (Catalog #MBS126233) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3K4me3 Polyclonal Antibody reacts with Human, Mouse, Rat, Other (Wide Range) and may cross-react with other species as described in the data sheet. MyBioSource\'s H3K4me3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP, CHIPseq.WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIP: 1:50 - 1:200. Researchers should empirically determine the suitability of the H3K4me3 hist3h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The H3K4me3 hist3h3 product has the following accession number(s) (GI #18202512) (NCBI Accession #Q16695.3) (Uniprot Accession #Q16695). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Histone H3K4me3 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K4 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Dot Blot (DB) (Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K4 antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human rectal cancer using TriMethyl-Histone H3-K4 antibody at dilution of 1:200 (40x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K4 antibody. Blue: DAPI for nuclear staining.)
Chromatin Imunoprecipitation (ChIP) (Chromatin immunoprecipitation analysis extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).