anti-GstS1 antibody product blog
Tags: Antibody; Polyclonal Antibody; Glutathione-S-Transferase [Schistosoma japonicum]; anti-GstS1 antibody; GstS1;
The GstS1 n/a (Catalog #MBS615407) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Glutathione-S-Transferase [Schistosoma japonicum] can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF).Suitable for immunomicroscopy, ELISA and Western Blot. Researchers should empirically determine the suitability of the GstS1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GstS1 n/a product has the following accession number(s) (GI #442623993) (NCBI Accession #NP_001261040.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, DYKDDDDK-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6 x His) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.
Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444D)
Absorption Wavelength: 550nm Emission Wavelength: 570nm. Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Goat IgG. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GstS1 are readily searchable from our website. Different antibodies against the same target such as GstS1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.