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anti-GSK3a (pS21) antibody product blog

Posted on 2015-07-18 12:00:50 by mybiosource_staff
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Tags: Antibody; GSK3a (pS21); anti-GSK3a (pS21) antibody;
The GSK3a (pS21) gsk3a (Catalog #MBS462105) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho- GSK-3alpha (pSer 21) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s GSK3a (pS21) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry.
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the GSK3a (pS21) gsk3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The GSK3a (pS21) gsk3a product has the following accession number(s) (GI #49574532) (NCBI Accession #NP_063937.2) (Uniprot Accession #P49840). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti Phospho- GSK-3alpha (pSer 21) with the following immunoassay(s):
Testing Data
Testing Data GSK3a (pS21).

Glycogen Synthase Kinase-3 has two isoforms, GSK-3alpha and GSK3beta. GSK3 is a serine/threonine kinase that affects glycogen metabolism by phosphorylating and down-regulating the activity of muscle glycogen synthase as well as the regulation in Wnt signal pathway. GSK-3 alpha reduces the substrates beta-catenin and tau to imply an important role in Wnt signaling and tauopathies such as Alzheimer\'s disease..

Antigen Preparation: A synthetic peptide surrounding to the epitope -RTSSFA- with a phosphorylation site at Ser21 of human GSK3-alpha protein. This sequence is identical among human, mouse and rat. Positive Control: 3T3 cell lysate
Cellular Location: Cytoplasmic. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GSK3a (pS21) are readily searchable from our website. Different antibodies against the same target such as GSK3a (pS21) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. GSK3a (pS21) also interacts with the following gene(s): AKT1, SGK1, SGK3. The following patways have been known to be associated with this gene. Blood, Brain, Connective Tissue, Embryonic Tissue, Heart, Kidney, Liver, Mammary Gland, Muscle, Ovary tissues are correlated with this protein. Adenocarcinoma, Adenoma, Cardiomegaly, Cardiovascular Diseases, Dementia, Fibrosis, Intestinal Diseases, Mental Disorders, Neoplasms, Neurodegenerative Diseases are some of the diseases may be linked to Rabbit anti Phospho- GSK-3alpha (pSer 21).
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