|The GRIN2B grin2b (Catalog #MBS502088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-Ser1480 NMDA Receptor NR2B Subunit reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NMDA NR2B Subunit (Ser1480) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Quality Control: Western blots performed on each lot.
WB: 1:1000. Researchers should empirically determine the suitability of the GRIN2B grin2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GRIN2B grin2b product has the following accession number(s) (GI #6980984) (NCBI Accession #NP_036706.1) (Uniprot Accession #Q00960). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Phospho-Ser1480 NMDA Receptor NR2B Subunit with the following immunoassay(s):
Affinity purified rabbit polyclonal antibody.
Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser1480 of the NR2B subunit of the rat NMDA receptor.
Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 conjugated to KLH
Immunogen Species: Rat. Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, non-human primates, and Xenopus
Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and zebrafish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance: The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer\'s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). It has been shown that phosphorylation of Ser1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GRIN2B are readily searchable from our website. Different antibodies against the same target such as GRIN2B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.