anti-GRIN1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-GRIN1 antibody; GRIN1; NMDA NR1 Subunit, Splice Variant C1;
The GRIN1 grin1 (Catalog #MBS502079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NMDA Receptor, NR1 Subunit, C1 Splice Variant Insert reacts with Mouse, rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NMDA NR1 Subunit, Splice Variant C1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC (frozen sections; unpublished observations): 1:1000 to 1:2000. Researchers should empirically determine the suitability of the GRIN1 grin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GRIN1 grin1 product has the following accession number(s) (GI #396578144) (NCBI Accession #NP_001257531.1) (Uniprot Accession #P35439). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-NMDA Receptor, NR1 Subunit, C1 Splice Variant Insert with the following immunoassay(s):
Testing Data
Affinity purified rabbit polyclonal antibody.
Antigen: Peptide from the NR1 subunit, C1 splice variant insert of the NMDA receptor.
Immunogen Information: Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C1 splice variant insert conjugated to KLH
Immunogen Species: Rat. Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, human, non-human primates
Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues. It is anticipated that the antibody will also work with bovine, canine, human, and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory and neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer\'s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2\'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.