anti-GRIA1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-GRIA1 antibody; GRIA1; GluR1;
The GRIA1 gria1 (Catalog #MBS624418) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GluR1, phosphorylated (Ser845) (Glutamate Receptor) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s GluR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA).Suitable for use in ELISA, Western Blot and Dot Blot.
Dilution: Western Blot: 1:1000
Dot Blot: 1:1000. Researchers should empirically determine the suitability of the GRIA1 gria1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GRIA1 gria1 product has the following accession number(s) (GI #167001484) (NCBI Accession #NP_001107655.1) (Uniprot Accession #P42261). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
In the mammalian central nervous system, glutamate and its receptors (GluR) are the major excitatory neurotransmitter and receptor, respectively. Imbalances in glutamatergic function have been implicated in neuronal death following ischemia, hypoglycemia or anoxia, epilepsy, and in neurodegenerative disorders. Based on their activation by different pharmacologic agonists GluR have been classified into two major superfamilies: the ligand-gated ion channel receptors referred to as ionotropic GluR (iGluR), and the G-protein-coupled receptors referred to as metabotropic GluR (mGluR). The iGluR respond to selective agonists and are thus defined as the N-methyl-D-aspartate (NMDA) receptors, the alphaamino-3-hydroxy-5-methyl-isoxasole-4-propionate (AMPA) responsive receptors (GluR1, 2, 3 and 4) and the kainate responsive receptors (GluR5 and 6). GluR1 is phosphorylated on multiple sites, all are located on the C-terminal of the protein. Ligand induced phosphorylation of GluR1 seems to be site-specific. In one study, using transfected HEK cells and neurons in culture, cyclic AMP-dependent protein kinase specifically phosphorylates Ser845 of GluR11. In addition, protein kinase C specifically phosphorylates Ser831 of GluR12. The phosphorylation pattern of GluR1 by PKA and PKC is consistent with the study of AMPA-Rs in cortical neurins and GluR1 expressed in 293 cells. These results suggested that the function of GluR1 can be regulated by protein phosphorylation.
Immunogen: Synthetic phospho-peptide derived from the rat GluR1 protein. Positive Control: Rat brain hippocampal homogenate. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GRIA1 are readily searchable from our website. Different antibodies against the same target such as GRIA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.