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anti-GPX1 antibody product blog

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Posted on 2013-09-17 02:48:48 by mybiosource_staff
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Tags: Antibody; GPX1; Polyclonal Antibody; anti-GPX1 antibody;
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The GPX1 gpx1 (Catalog #MBS125107) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPX1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s GPX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS).
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:20 - 1:50
FC: 1:20 - 1:50. Researchers should empirically determine the suitability of the GPX1 gpx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The GPX1 gpx1 product has the following accession number(s) (GI #311033481) (NCBI Accession #P07203.4) (Uniprot Accession #P07203). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GPX1 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of mouse liver, using GPX1 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB) GPX1.

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Species: Human
Route: Synthetic Peptide. Immunogen: A synthetic peptide of human GPX1
Calculated Molecular Weight: 22kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GPX1 are readily searchable from our website. Different antibodies against the same target such as GPX1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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