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anti-GOLM1 antibody product blog

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Posted on 2013-07-31 10:39:06 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; GOLPH2; anti-GOLM1 antibody; GOLM1;
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The GOLM1 golm1 (Catalog #MBS420062) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GOLPH2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. MyBioSource\'s GOLPH2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf), Immunohistochemistry (IHC).
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Prostate shows luminal staining of secretory cells in the glands. Similar results were obtained in Human Uterus and Small intestine. Recommended concentration, 3-6ug/ml.
Western Blot: In transfected HEK293 transiently expressing full-length Human GOLM1 (myc and DYKDDDDK tagged), a band of approx.75kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml. Researchers should empirically determine the suitability of the GOLM1 golm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The GOLM1 golm1 product has the following accession number(s) (GI #29550838) (NCBI Accession #NP_057632.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
NLLDQREKRN HTL.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-GOLPH2 Antibody with the following immunoassay(s):
Western Blot (WB) (HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human GOLM1 with C-terminal MYC tag probed (1ug/ml) in Lane An and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Western Blot (WB) GOLM1.

Immunohistochemistry (IHC) ((3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC) GOLM1.

Immunogen: Peptide with sequence C-NLLDQREKRNHTL, from the C Terminus of the protein sequence according to NP_057632.2; NP_808800.1.
Epitope: C Terminus. Note: Both variants (NP_057632.2; NP_808800.1) encode the same isoform. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GOLM1 are readily searchable from our website. Different antibodies against the same target such as GOLM1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. GOLM1 also interacts with the following gene(s): AFP, GOLPH3, GPC3.
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