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anti-GNAT3 antibody product blog

Posted on 2013-10-19 19:44:07 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; GNAT3; anti-GNAT3 antibody;
The GNAT3 gnat3 (Catalog #MBS421805) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GNAT3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. MyBioSource\'s GNAT3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC).
Peptide ELISA: Antibody detection limit dilution 1: 32000.

Western Blot: Preliminary experiments gave an approx 27kDa band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.4kDa according to NP_001095856.1. The 27kDa band was successfully blocked by incubation with the immunizing peptide.

Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of endocrine cells amongst the enterocytes. Recommended concentration, 3-6ug/ml. Researchers should empirically determine the suitability of the GNAT3 gnat3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The GNAT3 gnat3 product has the following accession number(s) (GI #156139155) (NCBI Accession #NP_001095856.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-GNAT3 Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) ((3.8ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC) GNAT3.

Immunogen: Peptide with sequence C-KNQFLDLNLKKEDKE, from the internal region of the protein sequence according to NP_001095856.1.
Epitope: Internal region. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GNAT3 are readily searchable from our website. Different antibodies against the same target such as GNAT3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Hyperinsulinism, Infertility, Male, Insulin Resistance, Obesity are some of the diseases may be linked to Goat anti-GNAT3 Antibody.
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