anti-GLUD1/GLUD2 antibody product blog
Tags: Antibody; Polyclonal Antibody; GLUTAMATE DEHYDROGENASE; anti-GLUD1/GLUD2 antibody; GLUD1/GLUD2;
The GLUD1 glud1 (Catalog #MBS422307) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Glutamate Dehydrogenase Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s Glutamate Dehydrogenase can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC).Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1). Recommended concentration: 0.3-1ug/ml.
Immunofluorescence: Customer found particulate cytoplasm staining in HeLa. Researchers should empirically determine the suitability of the GLUD1 glud1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GLUD1 glud1 product has the following accession number(s) (GI #4885281) (NCBI Accession #NP_005262.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
ESEEQKRNRV RGILR.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-Glutamate Dehydrogenase Antibody with the following immunoassay(s):
Western Blot (WB) ((0. 03ug/ml) staining of Human (A) and Mouse (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) ((0. 01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC) ((3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunogen: Peptide with sequence C-ESEEQKRNRVRGILR, from the internal region of the protein sequence according to NP_005262.1; NP_036216.2.
Epitope: Internal region. Note: This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GLUD1 are readily searchable from our website. Different antibodies against the same target such as GLUD1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.