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anti-GJA1 antibody product blog

Posted on 2014-02-15 07:10:08 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; GJA1; anti-GJA1 antibody; Connexin 43/GJA1;
The GJA1 gja1 (Catalog #MBS421720) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Connexin 43/GJA1 Antibody reacts with Tested: Rat; Expected from sequence similarity: Human, Rat, Dog, Pig and may cross-react with other species as described in the data sheet. MyBioSource\'s Connexin 43/GJA1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 40kDa band observed in Rat Brain lysates (calculated MW of 43. 0kDa according to Human NP_000156.1 and to Rat NP_036699.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the GJA1 gja1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The GJA1 gja1 product has the following accession number(s) (GI #4504001) (NCBI Accession #NP_000156.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-Connexin 43/GJA1 Antibody with the following immunoassay(s):
Western Blot (WB) ((0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) GJA1.

Immunogen: Peptide with sequence C-QPFDFPDDNQNSKK, from the internal region of the protein sequence according to NP_000156.1.
Epitope: Internal region. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GJA1 are readily searchable from our website. Different antibodies against the same target such as GJA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Arrhythmias, Cardiac, Breast Neoplasms, Disease Models, Animal, Fibrosis, Heart Diseases, Inflammation, Lung Neoplasms, Necrosis, Neoplasms, Experimental, Oculodentodigital Dysplasia are some of the diseases may be linked to Goat anti-Connexin 43/GJA1 Antibody. GJA1 also interacts with the following gene(s): CDH2, CTNNB1, JUN, MAPK1, SRC, TJP1, UBC, UBQLN4. The following patways have been known to be associated with this gene. Bone, Brain, Embryonic Tissue, Eye, Heart, Nerve, Ovary, Skin, Uterus, Vascular tissues are correlated with this protein.
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