anti-GFP antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-GFP antibody; GFP;
The GFP gfp (Catalog #MBS534795) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The GFP antibody (biotin) reacts with Assay by immunoelectrophoresis resulted in a single precipitation arc against anti-goat serum, anti-biotin and purified and partially purified green fluorescent protein (Aequorea victoria) Serum. no reaction was observed against Human, Mouse, and Rat ser and may cross-react with other species as described in the data sheet.ELISA: 1:10,000-1:50,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000
Immunomicroscopy as well as other antibody-based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Researchers should empirically determine the suitability of the GFP gfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GFP gfp product has the following accession number(s) (GI #1169893) (NCBI Accession #P42212.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GFP antibody (biotin) with the following immunoassay(s):
Immunofluorescence (IF) (issue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.)
Immunofluorescence (IF) (Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen retrieval: not required. Anti-GFP antibody at a 1:1,000 for 1 h at RT. AlexaFluor 488 conjugated anti-Goat antibody at 1:300 for 45 min at RT. Panel A: shows a lateral view (ventral left). Panels B and C: shows ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). In all panels, anterior is up. Staining: tau-GFP cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) as green fluorescent signal.)
Western Blot (WB) (Western blot of Biotin GFP antibody. Lane 1: GFP. Lane 2: None. Load: 50 ng per lane. GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for GFP.)
Western Blot (WB) (Western Blot of GFP antibody. Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. GFP antibody at 1 ug/ml for 1 h at room temperature. IRDye 800 conjugated Donkey-a-Goat IgG [H&L] MX7 (605-732-125) secondary antibody at 1:2,500 for 45 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: 27 kDa, 33 kDa for GFP.)
Goat polyclonal GFP antibody (biotin) conjugated.