|The GAD2 gad2 (Catalog #MBS618215) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glutamate Decarboxylase 65,67 (GAD 65,67) reacts with Feline, Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Glutamate Decarboxylase 65,67 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
Suitable for use in Immunohistochemistry, ELISA, Western Blot and Immunofluorescence.
Dilution: Immunohistochemistry (Frozen): 1:200-1:5000
Immunohistochemistry: Paraffin sections
Western Blot: 1:1000-1:10,000. A doublet at ~65/67kD is expected.
Optimal working dilutions to be determined by researcher.
Recommended Positive Control Tissues: Brain. Researchers should empirically determine the suitability of the GAD2 gad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GAD2 gad2 product has the following accession number(s) (GI #4503875) (NCBI Accession #NP_000809.1) (Uniprot Accession #Q05329). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Glutamic acid decarboxylase (GAD; E.C. 184.108.40.206) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65kD and 67kD, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585aa) encoded on human chromosome10. GAD67 is cytoplasmic (594aa) and is encoded on chromosome 2. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, P. et al. 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, L. et al. 1995). Changes in GAD65/GAD67 distributions have also been correlated with certain disease states such as IDDM and SMS.
Immunogen: Synthetic peptide corresponding to DFLIEEIERLGQDL from the C-terminus of rat GAD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GAD2 are readily searchable from our website. Different antibodies against the same target such as GAD2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.