anti-FZD8 antibody product blog
Tags: Antibody; Polyclonal Antibody; FZD8/frizzled 8; anti-FZD8 antibody; FZD8;
The FZD8 fzd8 (Catalog #MBS420018) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FZD8/frizzled 8 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s FZD8/frizzled 8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Pancreas, shows granular cytoplasmic staining of acinar cells. Cells of the Islets of Langerhans are weakly stained, if at all. Recommended concentration, 3ug/ml.
Western Blot: Approx 75kDa band observed in human heart and human pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the FZD8 fzd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The FZD8 fzd8 product has the following accession number(s) (GI #13994190) (NCBI Accession #NP_114072.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
SYPKQMPLSQ V.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-FZD8/frizzled 8 Antibody with the following immunoassay(s):
Western Blot (WB) ((1ug/ml) staining of human pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC) ((3ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-)
Immunogen: Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1.
Epitope: C Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FZD8 are readily searchable from our website. Different antibodies against the same target such as FZD8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Bone, Brain, Embryonic Tissue, Eye, Heart, Kidney, Lung, Pancreas, Testis, Uterus tissues are correlated with this protein. Abnormalities, Drug-Induced, Breast Neoplasms, Disease Models, Animal, Fibrosis, Hypertension, Inflammation, Kidney Diseases, Lung Neoplasms, Prenatal Exposure Delayed Effects, Prenatal Injuries are some of the diseases may be linked to Goat anti-FZD8/frizzled 8 Antibody.