anti-FSCN1 antibody product blog
Tags: Antibody; Polyclonal Antibody; Fascin; FSCN1; anti-FSCN1 antibody;
The FSCN1 fscn1 (Catalog #MBS175408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Fascin antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Fascin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the FSCN1 fscn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The FSCN1 fscn1 product has the following accession number(s) (GI #2498357) (NCBI Accession #Q16658.3) (Uniprot Accession #Q16658). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Fascin antibody with the following immunoassay(s):
Western Blot (WB) (Anti-Fascin antibody, MBS175408, Western blotting
All lanes: Anti Fascin (MBS175408) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD )
Description: Rabbit IgG polyclonal antibody for Fascin(FSCN1) detection. Tested with WB in Human, Mouse, Rat.
Background: Fascin is an actin cross-linking protein. The Fascin gene contains 5 exons and spans 7 kb. It is a 54-58 kilodalton monomeric actin filament bundling protein originally isolated from sea urchin egg but also found in Drosophila and vertebrates, including humans. Fascin(from the Latin for bundle) is spaced at 11 nanometre intervals along the filament. The bundles in cross section are seen to be hexagonally packed, and the longitudinal spacing is compatible with a model where fascin cross-links at alternating 4 and 5 actins. It is calcium insensitive and monomeric. Fascin binds beta-catenin, and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of Fascin in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. Abnormal fascin expression or function has been implicated in breast cancer, colon cancer, esophageal squamous cell carcinoma, gallbladder cancer and prostate cancer.
Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Fascin (97-113aa DDGRWSLQSEAHRRYFG), identical to the related rat and mouse sequences. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FSCN1 are readily searchable from our website. Different antibodies against the same target such as FSCN1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.