anti-FOXI1 antibody product blog
Tags: Antibody; Polyclonal Antibody; FOXI1/FKHL10; anti-FOXI1 antibody; FOXI1;
The FOXI1 foxi1 (Catalog #MBS420822) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FOXI1/FKHL10 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. MyBioSource\'s FOXI1/FKHL10 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC).Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Testis shows primarily nuclear staining in the primary spermatocytes. Recommended concentration, 2-4ug/ml. This product has been successfully used in Mouse Epididymus by anonymous customer.
Western Blot: Preliminary experiments gave bands at approx 50 kDa and 25 kDa in Human Kidney lysate after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted sizes of 41. 0kDa according to NP_036320 (isoform a) and 30.8kDa according to NP_65892 (isofrom b). The bands were successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the FOXI1 foxi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The FOXI1 foxi1 product has the following accession number(s) (GI #21618327) (NCBI Accession #NP_036320.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
TSGVLYPREG TEV.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-FOXI1/FKHL10 Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) ((2ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunogen: Peptide with sequence C-TSGVLYPREGTEV, from the C Terminus of the protein sequence according to NP_036320.2; NP_658982.1.
Epitope: C Terminus. Note: This antibody is expected to recognise both reported human isoforms (NP_036320.2; NP_658982.1). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FOXI1 are readily searchable from our website. Different antibodies against the same target such as FOXI1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Kidney, Lung tissues are correlated with this protein. Chromosome Aberrations, Craniofacial Abnormalities, DEAFNESS, AUTOSOMAL RECESSIVE 4, WITH ENLARGED VESTIBULAR AQUEDUCT, Disease Models, Animal, Fibrosis, Hypertension, Infertility, Female, Kidney Diseases, Liver Neoplasms, Nervous System Diseases are some of the diseases may be linked to Goat anti-FOXI1/FKHL10 Antibody.