Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow anti-FOXA1 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • MYLK elisa kit blog
 • p15 Treponema Pallidum recombinant protein blog
 • PLGA elisa kit blog
 • PDIA3 blocking peptide blog
 • anti-GAA antibody blog
 • WT1 peptide blog
 • MID1 shrna blog
 • FIS1 sirna blog
 • VCX2 sirna blog
 • IgG secondary antibody blog
 • MAB21L2 elisa kit blog
 • CHGA blocking peptide blog
 • anti-PGAM1/PGAM2/PGAM4 antibody blog
 • anti-H antibody blog
 • anti-a-Melanocyte Stimulating Hormone antibody blog
 • rpsH recombinant protein blog
 • SAG elisa kit blog
 • CEP85L sirna blog
 • LARP1B blocking peptide blog
 • AG elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • IL-1alpha ELISA Kit
 • Alanine Aminotransferase (GPT) Protein
 • carbonic anhydrase IX (CA9) ELISA Kit
 • CAM ELISA Kit
 • Alpha 2-Macroglobulin (A2M) Reference Standard Reference Serum & Calibrator
 • Human Chorionic Gonadotropin (hCG), Free beta Antibody
 • Erythropoietin (EPO) Recombinant Protein
 • Helix aspersa Lectin
 • APEX1 Antibody
 • RO 20-1724, cAMP-specific Phosphodiesterase Inhibitor Biochemical
 • MICU1 Antibody
 • Hyaluronidase (HAase) ELISA Kit
 • 12-LO ELISA Kit
 • AFGF-FGF1 ELISA Kit
 downarrow more ...
separator

anti-FOXA1 antibody product blog

separator
Posted on 2014-07-30 00:25:59 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; Polyclonal Antibody; FOXA1; anti-FOXA1 antibody; FOXA1/HNF3A;
separator
The FOXA1 foxa1 (Catalog #MBS420148) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FOXA1/HNF3A Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s FOXA1/HNF3A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP).
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Prostate shows preferential nuclear staining in the epithelial cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 50kDa band observed in lysates of cell line HepG2 (calculated MW of 49.1kDa according to NP_004487.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide.
Chromatin Immunoprecipitation: This antibody has been successfully used for mouse embryonic stem cells and it has been confirmed in ChIP by anonymous customers. Researchers should empirically determine the suitability of the FOXA1 foxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The FOXA1 foxa1 product has the following accession number(s) (GI #24497501) (NCBI Accession #NP_004487.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
GVYSRPVLNT S.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-FOXA1/HNF3A Antibody with the following immunoassay(s):
Western Blot (WB) ((0.3ug/ml) staining of lysates of cell line HepG2 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) FOXA1.

Immunogen: Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.
Epitope: C Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FOXA1 are readily searchable from our website. Different antibodies against the same target such as FOXA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. FOXA1 also interacts with the following gene(s): ESR1, FOXA2, INS, NKX2-1, NKX3-1, SCGB1A1, SHH, TFF1, TTR.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions