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anti-FOXA1 antibody product blog

Posted on 2014-07-30 00:25:59 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; FOXA1; anti-FOXA1 antibody; FOXA1/HNF3A;
The FOXA1 foxa1 (Catalog #MBS420148) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FOXA1/HNF3A Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s FOXA1/HNF3A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP).
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Prostate shows preferential nuclear staining in the epithelial cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 50kDa band observed in lysates of cell line HepG2 (calculated MW of 49.1kDa according to NP_004487.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide.
Chromatin Immunoprecipitation: This antibody has been successfully used for mouse embryonic stem cells and it has been confirmed in ChIP by anonymous customers. Researchers should empirically determine the suitability of the FOXA1 foxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The FOXA1 foxa1 product has the following accession number(s) (GI #24497501) (NCBI Accession #NP_004487.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-FOXA1/HNF3A Antibody with the following immunoassay(s):
Western Blot (WB) ((0.3ug/ml) staining of lysates of cell line HepG2 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) FOXA1.

Immunogen: Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.
Epitope: C Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FOXA1 are readily searchable from our website. Different antibodies against the same target such as FOXA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. FOXA1 also interacts with the following gene(s): ESR1, FOXA2, INS, NKX2-1, NKX3-1, SCGB1A1, SHH, TFF1, TTR. Bladder, Brain, Embryonic Tissue, Intestine, Kidney, Liver, Lung, Mammary Gland, Nerve, Prostate tissues are correlated with this protein.
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