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anti-FLT-1/VEGFR1 antibody product blog

Posted on 2015-05-11 01:30:20 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; FLT-1/VEGFR1; anti-FLT-1/VEGFR1 antibody;
The FLT-1/VEGFR1 flt1 (Catalog #MBS301250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human Flt-1/VEGFR1 Polyclonal Antibody reacts with Human
Predicted Reactivity: Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s FLT-1/VEGFR1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin.
Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.cooling at room temperature for 20 min. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:50
IHC Positive Control: Placenta, Angiosarcoma. Researchers should empirically determine the suitability of the FLT-1/VEGFR1 flt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The FLT-1/VEGFR1 flt1 product has the following accession number(s) (GI #229892220) (NCBI Accession #NP_001153392.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Anti-Human Flt-1/VEGFR1 Polyclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Human placenta stained with anti-Flk-1 antibody)
Immunohistochemistry (IHC) FLT-1/VEGFR1.

Flt-1/vascular endothelial growth factor receptor 1 (VEGFR1), a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to Flk-1 and Flt-4.

Immunogen: Synthetic peptide derived from C-terminus of mouse FLK-1.
Control Tissue: Angiosarcoma, Placenta
Cellular Localization: Cytoplasm, Membrane
Entrez Gene Code: 2321
Pathway: Angiogenesis. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FLT-1/VEGFR1 are readily searchable from our website. Different antibodies against the same target such as FLT-1/VEGFR1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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