anti-fliP antibody product blog
Tags: Antibody; Polyclonal Antibody; FLIP; anti-FLIP antibody; FLIP, cellular;
The fliP flip (Catalog #MBS611022) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FLIP, cellular (cFLIP, FLICE Inhibitory Protein) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s FLIP, cellular can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).Western Blot: 0.5-1ug/ml, Immunocytochemistry: 5ug/ml, Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the fliP flip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The fliP flip product has the following accession number(s) (GI #25166005) (Uniprot Accession #Q8D3F1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
cFLIP (cellular FLICE-like inhibitory protein) is a cellular homologue of the viral protein, vFLIP, and is also known as CASH, Casper, CLARP, FLAME, I-FLICE, MRIT and Usurpin (2,3). cFLIP contains two death effector domains (DEDs) and is expressed as either a short or long splice variant called cFLIPs and cFLIPL, respectively (3,4). cFLIP can block Fas-mediated apoptosis presumably through the C-terminal region of cFLIPL which has homology with the proteolytic domain of caspase-8 (FLICE). However, cFLIP lacks the active proteinase site required for catalytic activity. Therefore, although it can be recruited to the ligated Fassignalling complex, it cannot deliver a death signal. cFLIP is expressed in the cytosol and it has been reported that cells with high levels of cFLIP relative to FLICE are generally resistant to apoptosis, whereas cells with low levels of cFLIP relative to FLICE are more sensitive to apoptosis (5). cFLIP acts as a caspase-8 dominant negative regulator, blocking apoptosis induced by the oligimerization of the adapter protein, FADD (5). cFLIP interacts with FADD and caspase-8 through their respective DEDs (2). The regulation and role of cFLIP in mediating signals that promote survival such as NF-kB versus inhibiting cell death by blocking cell death receptor-induced caspase activation is unclear (6).
Immunogen: A 18 residue synthetic peptide based on the amino terminus of human c-FLIP (residues 2-19) was synthesized and the peptide coupled to KLH. This sequence is identical in all c-FLIP splice variants. The amino acid sequence is approximately 90% identical to that of mouse. Positive Control: HeLa Cell Lysate. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing fliP are readily searchable from our website. Different antibodies against the same target such as fliP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.