|The FKBP52 n/a (Catalog #MBS190476) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s FKBP52 can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunohistochemistry, Immunoprecipitation.
Immunoblotting: use at 0.5-1ug/ml. A band of ~52 kDa is detected.
Immunohistochemistry: use at 4-5ug/ml
Immunoprecipitation: 5ug on 10-20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue extracts. Researchers should empirically determine the suitability of the FKBP52 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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FK506-binding proteins, FKBP51 and FKBP52, are high molecular weight immunophilins that are part of the mature glucocorticoid receptor heterocomplex. The N-terminal domains of these proteins bind FK506 and have peptidyl-prolyl isomerase activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the tetratricopeptide repeats that are involved in protein-protein interactions with Hsp90. Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptors differently and have different Hsp90-binding characteristics. In addition, FKBP51 is linked to the progesterone receptor while FKBP52 is linked to progesterone, androgen, and glucocorticoid receptors.
Antigen: Recombinant full-length FKBP52 with an N-terminal polyhistidine tag. Preservatives: 0.09% sodium azide
Other Additives: 50% glycerol
Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FKBP52 are readily searchable from our website. Different antibodies against the same target such as FKBP52 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.