anti-filaggrin antibody, AFA elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; anti-filaggrin antibody, AFA; anti-filaggrin antibody, AFA elisa kit;
The Human anti-filaggrin antibody, AFA n/a (Catalog #MBS704877) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS704877 ELISA Kit recognizes Human anti-filaggrin antibody, AFA.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human anti-filaggrin antibody, AFA ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with filaggrin antigen. Samples are then added to the appropriate microtiter plate wells and incubated. Then add Horseradish Peroxidase (HRP)-conjugated anti-human IgG and incubate again. Substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. Calculate the valence of AFA in the samples.
Samples: Serum. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Human anti-filaggrin antibody, AFA ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.