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anti-FABP4 antibody product blog

Posted on 2014-10-23 03:22:37 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; FABP4; anti-FABP4 antibody;
The FABP4 fabp4 (Catalog #MBS200120) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FABP4 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s FABP4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS).
WB: 1:500 ~ 1,000.
Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the FABP4 fabp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The FABP4 fabp4 product has the following accession number(s) (GI #4557579) (NCBI Accession #NP_001433) (Uniprot Accession #P15090). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the FABP4 antibody with the following immunoassay(s):
Western Blot (WB) (The extracts of mouse heart (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FABP4 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The extracts of mouse adipose (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB) FABP4.

FABP4 (Fatty acid binding protein 4) is expressed in adipocytes and macrophages, and integrates inflammatory and metabolic responses. FABP4 is thought to regulate fatty acid uptake, release, and storage in adipocytes and participates in systemic glucose homeostasis and in macrophage responses in atherosclerosis. Blocking this protein either through genetic engineering or drugs has the possibility of treating heart disease, diabetes, asthma, obesity, and fatty liver disease.

Antigen Species: Human
Immunogen: Recombinant human FABP4 (1-132aa) purified from E. coli. Clone Note: Anti-human FABP4 mAb, clone 3F4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FABP4 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FABP4 are readily searchable from our website. Different antibodies against the same target such as FABP4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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