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anti-F8 antibody product blog

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Posted on 2014-07-15 05:26:58 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-F8 antibody;
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The F8 f8 (Catalog #MBS850307) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The F8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s F8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).
WB: 1/500 - 1/2000, ELISA: Propose dilution 1/10000
Not yet tested in other applications. Determining optimal working dilutions by titration test. Researchers should empirically determine the suitability of the F8 f8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The F8 f8 product has the following accession number(s) (GI #4503647) (NCBI Accession #NP_000123.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the F8 Antibody with the following immunoassay(s):
Testing Data (Figure 1. Western blot analysis using F8 mouse mAb against truncated Trx-F8 recombinant protein (1).)
Testing Data F8.

Background: F8. coagulation factor VIII, procoagulant component. This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

Immunogen: Purified recombinant fragment of F8 expressed in E. Coli.
Species: Mouse IgG1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing F8 are readily searchable from our website. Different antibodies against the same target such as F8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Heart, Kidney, Liver, Lung, Muscle, Nerve, Skin, Vascular tissues are correlated with this protein. F8 also interacts with the following gene(s): F10, LMAN1, SERPINE1, VWF. The following patways have been known to be associated with this gene.
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