|The F5 f5 (Catalog #MBS512038) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase.
Suitable as a source of antibodies to human factor V. Researchers should empirically determine the suitability of the F5 f5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The F5 f5 product has the following accession number(s) (GI #182798) (Uniprot Accession #P12259). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Factor V (formerly referred to as accelerator globulin and labile factor) is a large glycoprotein (320 kDa) that is produced in the liver. The gene that encodes factor V (F.V) is located on chromosome 1. A congenital deficiency of F.V is a hemorrhagic disorder inherited as an autosomal recessive disease. The concentration of F.V in plasma is typically 10 ug/ml. F.V is a pro-cofactor that is activated through limited proteolysis by thrombin, or by activated factor X in the presence of phospholipid surface. Other physiologic activators of F.V include plasmin, neutrophil elastase and platelet calpain. The activated cofactor (F.Va) is an essential component of the prothrombin activator complex, which consists of F.Va, activated factor X, calcium and anionic phospholipid surface. The intact prothrombinase complex activates prothrombin to thrombin at a rate 300,000-fold greater than activated factor X alone. In a positive feedback loop, the thrombin generated accelerates its own generation by activating more F.V to F.Va. Thrombin also acts to down-regulate F.Va indirectly by activating Protein C, which inactivates F.Va cofactor activity1-3.
Immunogen: Human factor V purified from plasma. Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
Neutralizing Activity: XXXX Bethesda Units/ml IgG against normal plasma. One Bethesda unit/ml is defined as the amount of inhibitor that resulted in 50% residual F.V activity after 2 hours at 37 degree C (Kasper CK et al, Thromb Diath Haemorrh 34:869, 1975). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing F5 are readily searchable from our website. Different antibodies against the same target such as F5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. F5 also interacts with the following gene(s): F10, PROC.