anti-F4/80, Pan Macrophages antibody product blog
Tags: Antibody; Monoclonal Antibody; F4/80, Pan Macrophages; anti-F4/80, Pan Macrophages antibody;
The F4/80, Pan Macrophages n/a (Catalog #MBS605325) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s F4/80, Pan Macrophages can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry.
Dilution: Flow Cytometry: 1:50, Fix with 1% paraformaldehyde (3).
Western Blot: 1:50, Mouse bone-marrow derived macrophages; non-reduced. Detects a band at ~125kD (3). Reduction with 2-mercaptoethanol destroys the antigen.
Immunohistochemistry (frozen): 1:50, Tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT. Incubation with 0.02M sodium azide in PBS, 0.1% H2O2 for 10min at RT to destroy endogenous peroxidase. Use spleen as positive control
Immunohistochemistry (paraffin): 1:50, Fixation in 10% neutral buffered formalin for 24 hours; blocking with non-immunized goat serum; microwaved for 6min in citrate buffer. Use splenic macrophages as positive control (5). Researchers should empirically determine the suitability of the F4/80, Pan Macrophages n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (FITC) with the following immunoassay(s):
Testing Data (F4/80 expression on macrophages in mouse liver. Staining of frozen tissue section with MBS605325.)
Mouse F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and trombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny.
Immunogen: BALB/c macrophages obtained from 14-day-old bone marrow cell cultures. Positive Control: Mouse macrophages. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing F4/80, Pan Macrophages are readily searchable from our website. Different antibodies against the same target such as F4/80, Pan Macrophages may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.