anti-F4/80 antibody product blog
Tags: Antibody; Monoclonal Antibody; F4/80; anti-F4/80 antibody;
The F4/80 emr1 (Catalog #MBS219371) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s F4/80 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Immunoelectron Microscopy (EM), Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Radioimmunoassays (RIA), Immunohistology Resin (RE).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Paraffin: Application Note: F4/80 antibody requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Researchers should empirically determine the suitability of the F4/80 emr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The F4/80 emr1 product has the following accession number(s) (GI #33859546) (NCBI Accession #NP_034260.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE F4/80 with the following immunoassay(s):
Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC (MBS216414))
Testing Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 (MBS216424), red and Rat anti Mouse CD45, green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)
Testing Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC (MBS216408))
Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750 (MBS219371P750))
Testing Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin (MBS216412))
Testing Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP (MBS235195) or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic MBS219371 (HCA154) followed by Goat anti Rat IgG:HRP (MBS235195))
Testing Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin (MBS216409))
Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue(MBS219371PB))
Testing Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)
Testing Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin (MBS216410))