anti-F2RL1 antibody product blog
Tags: Antibody; PAR2; Monoclonal Antibody; anti-F2rl1 antibody; F2rl1;
The F2RL1 f2rl1 (Catalog #MBS601417) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PAR2 (Proteinase Activated Receptor 2) (PE) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PAR2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: Use 10ul to label 1x10e5 cells or 50ul of packed whole blood. Researchers should empirically determine the suitability of the F2RL1 f2rl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The F2RL1 f2rl1 product has the following accession number(s) (GI #34577052) (NCBI Accession #NP_005233.3) (Uniprot Accession #P55085). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PAR2 (Proteinase Activated Receptor 2) (PE) with the following immunoassay(s):
Testing Data (HT-29 cells were stained with MBS601417 (filled histogram) or isotype control (open histogram).)
Human protease-activated receptor 2 (PAR2) is one of 4 members in an unique subclass of the Class A G protein- coupled receptor superfamily. Receptor activation involves the proteolytic uncovering of a cryptic N-terminus receptor sequence that forms a tethered ligand and binds to the receptors within the extracellular loop 2. This activates the receptors to initiate multiple signaling cascades. In addition, short peptides (5-6 amino acids) synthesized based on the proteolytically revealed motif can activate the receptors without the cleavage by enzymes. PAR2 is activated by trypsin and trypsin-like enzymes, and the cleavage site has been identified at residue Arg 36. PAR2 plays an important role in the regulation of cardiovascular, gastrointestinal, and skin function. PAR2 is expressed in human vascular tissues, pancreas, kidney, colon, liver, small intestine, and some tumor cell lines (1, 2).
Immunogen: NS0 mouse myeloma cell line transfected with human PAR2, Accession # P55085. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing F2RL1 are readily searchable from our website. Different antibodies against the same target such as F2RL1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.