anti-Erythroid Cell (Ly 76) antibody product blog
Tags: Antibody; Monoclonal Antibody; Erythroid Cell (Ly 76); anti-Erythroid Cell (Ly 76) antibody;
The Erythroid Cell (Ly 76) n/a (Catalog #MBS520283) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse Erythroid Cell (Ly 76), Biotin (Clone TER119) (rat IgG2b) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Erythroid Cell (Ly 76) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the Erythroid Cell (Ly 76) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Mouse Erythroid Cell (Ly 76), Biotin (Clone TER119) (rat IgG2b) with the following immunoassay(s):
Testing Data
anti-mouse erythroid cell (Ly-76) mAb is selectively reactive with both fetal and adult erythroid cells. This monoclonal antibody (clone: TER119) is specific for cells at stages from early proerythroblast to mature erythrocytes. TER119 is reported to react with 20-25% of bone marrow cells and 2-3% of spleen cells but not with thymocytes or lymph node cells. In fetal haematopoietic tissues, 30-40% of day 10 yolk sac cells, 80-90% of day 14 fetal liver cells and 40-50% of newborn liver cells were reactive. TER119+ cells in adult bone marrow expressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B cell (B220) markers. This mAb immunoprecipitated protein bands with molecular weights of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane whereas only a 52 kDa band was detected by TER119 in Western blot analysis. It has been determined that the TER119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin An itself. Also the antigen is only expressed on normal erythroid cells but not on erythroleukaemia cells. This antibody is suitable for use in flow cytometry. It has also be reported to work in Western Blotting, immunoprecipitation and immunohistochemistry (frozen and paraffin).
Immunogen: Day 14 BALB/c fetal liver cells
Donor: Wistar rat spleen
Fusion Partner: X63.Ag8.653 myloma cells. Presentation: 100 ug or 300ug Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Erythroid Cell (Ly 76) are readily searchable from our website. Different antibodies against the same target such as Erythroid Cell (Ly 76) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.