anti-ERO1A antibody product blog
Tags: Antibody; Polyclonal Antibody; ERO1A / ERO1L; anti-ERO1A antibody; ERO1A;
The ERO1A ero1l (Catalog #MBS243472) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human ERO1A / ERO1L reacts with Bat, Gibbon, Dog, Gorilla, Human, RabbitPredicted Reactivity: Monkey, Mouse, Rat, Bovine, Horse, Pig (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s ERO1A / ERO1L can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA (1:32000), IHC-P (5 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 70kD band observed in lysates of cell lines A431, HeLa, Jurkat (calculated MW of 54.4kD according to NP_055399.1). The observed molecular weight corresponds to the glycosy.. Researchers should empirically determine the suitability of the ERO1A ero1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ERO1A ero1l product has the following accession number(s) (GI #7657069) (NCBI Accession #NP_055399.1) (Uniprot Accession #Q96HE7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat Polyclonal to Human ERO1A / ERO1L with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-ERO1L antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB) (ERO1L antibody (0.3 ug/ml) staining of A431 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Target Species: Human
Immunogen Description: Synthetic peptide C-QSDEVPDGIKSASY from an internal region of human ERO1L (NP_055399.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog, Bat, Rabbit (100%); Marmoset, Mouse, Rat, Bovine, Horse, Pig (93%). Immunogen Type: Synthetic peptide
Immunogen: ERO1A / ERO1L antibody was raised against synthetic peptide C-QSDEVPDGIKSASY from an internal region of human ERO1L (NP_055399.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog, Bat, Rabbit (100%); Marmoset, Mouse, Rat, Bovine, Horse, Pig (93%); Turkey, Platypus (86%).
Antigen Modification: Internal. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ERO1A are readily searchable from our website. Different antibodies against the same target such as ERO1A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.