anti-ERK3 antibody product blog
Tags: Antibody; anti-ERK3 antibody; ERK3; Monoclonal Antibody;
The ERK3 mapk6 (Catalog #MBS850582) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ERK3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ERK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA).WB: 1:500-1:2000
FC: 1:200-1:400
ELISA: 1:10000. Researchers should empirically determine the suitability of the ERK3 mapk6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ERK3 mapk6 product has the following accession number(s) (GI #4506091) (NCBI Accession #NP_002739.1) (Uniprot Accession #Q16659). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ERK3 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using ERK3 mAb against HEK293 (1) and ERK3-hIgGFc transfected HEK293 (2) cell lysate.)
Flow Cytometry (FC/FACS) (Figure 2: Flow cytometric analysis of Hela cells using ERK3 mouse mAb (green) and negative control (purple).)
Testing Data
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Immunogen: Purified recombinant fragment of human ERK3 expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ERK3 are readily searchable from our website. Different antibodies against the same target such as ERK3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Brain, Embryonic Tissue, Eye, Kidney, Lung, Muscle, Prostate, Skin, Vascular tissues are correlated with this protein. ERK3 also interacts with the following gene(s): MAPKAPK5. Carcinoma, Carcinoma, Squamous Cell, Cardiovascular Diseases, Cell Transformation, Neoplastic, Death, Heart Diseases, Immune System Diseases, Inflammation, Necrosis, Neoplasm Metastasis are some of the diseases may be linked to ERK3 Antibody.