anti-ER (pS305) (phosphospecific) antibody product blog
Tags: Antibody; ER (pS305) (phosphospecific); anti-ER (pS305) (phosphospecific) antibody;
The ER (pS305) (phosphospecific) n/a (Catalog #MBS462046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti ER (pSer 305), Phosphospecific reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ER (pS305) (phosphospecific) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot.Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:200. Researchers should empirically determine the suitability of the ER (pS305) (phosphospecific) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti ER (pSer 305), Phosphospecific with the following immunoassay(s):
Testing Data
ER (estrogen receptor) belongs to a member of the steroid receptor superfamily. It contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by its phosphorylation at multiple sites, such as serine 104, 106, 118, 167 and 305. Phosphorylation of certain serine at ER may relate to breast cancer process and on-going treatment.
Antigen Preparation: A synthetic peptide surrounding the epitope -KNSLA- with a phosphorylation site of Ser305 of human estrogen receptor. Positive Control: MCF-7
Cellular Location: Nuclear. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ER (pS305) (phosphospecific) are readily searchable from our website. Different antibodies against the same target such as ER (pS305) (phosphospecific) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.