anti-EPO antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-EPO antibody; EPO;
The EPO epo (Catalog #MBS531810) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EPO antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EPO can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the EPO epo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The EPO epo product has the following accession number(s) (GI #312304) (NCBI Accession #CAA26095.1) (Uniprot Accession #P01588). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EPO antibody with the following immunoassay(s):
ELISA (EIA) (EPO concentration was determined to be in the range of 2-200 ng/mL. Antibody used at 5 ug/mL to coat plate. Optimal working dilutions must be determined by the end user.)
Immunofluorescence (IF) ()
Immunofluorescence (IF) ()
Western Blot (WB) (Mouse kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-EPO (1:500 dilution). Arrow indicates glycosylated EPO (~34 kDa).)
Mouse monoclonal EPO antibody.
Biological Significance: Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia. Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.
Immunogen: EPO antibody was raised in mouse using recombinant human EPO as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EPO are readily searchable from our website. Different antibodies against the same target such as EPO may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. EPO also interacts with the following gene(s): EPAS1, EPOR, HIF1A, JAK1, JAK2, KITLG, SIRT1, SOCS3, STAT5A, STAT5B. The following patways have been known to be associated with this gene.