anti-EphA2 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-EPHA2 antibody; EPHA2;
The EphA2 epha2 (Catalog #MBS850883) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EphA2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EphA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA).WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000. Researchers should empirically determine the suitability of the EphA2 epha2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EphA2 epha2 product has the following accession number(s) (GI #32967311) (NCBI Accession #NP_004422.2) (Uniprot Accession #P29317). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EphA2 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1. Western blot analysis using EphA2 mouse mAb against NIH/3T3 cell lysate.)
Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human skin carcinoma (left) and pancreas carcinoma (right) tissue, showing cytoplasmic localization using EphA2 mouse mAb with DAB staining.)
EPH receptor A2 (EphA2), with 976-amino acid protein (about 107 kDa), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphA10, EphB1, EphB2, EphB3, EphB4 and EphB6 are Eph family receptors for Ephrin family ligands. In normal cells, EphA2 negatively regulates cell growth and invasiveness. EphA2 is overexpressed by many human cancers, and is often associated with poor prognostic features. The clinical significance of the expression of EphA2 was observed in breast, prostate, colon, skin, cervical, ovarian, and lung cancers.EphA2 may serve as a novel target for bladder cancer, colonic adenocarcinoma and ovarian cancer therapy.
Immunogen: Purified recombinant fragment of EphA2 expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EphA2 are readily searchable from our website. Different antibodies against the same target such as EphA2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Brain Diseases, Breast Neoplasms, Carcinoma, Squamous Cell, Disease Models, Animal, Lung Diseases, Lung Neoplasms, Neoplasms, Neoplasms, Experimental, Nervous System Diseases, Ovarian Neoplasms are some of the diseases may be linked to EphA2 Antibody.