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anti-ELF3 antibody product blog

Posted on 2013-11-26 09:15:13 by mybiosource_staff
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Tags: Antibody; ELF3; anti-ELF3 antibody; Polyclonal Antibody; ELF3/ERT/ ESX;
The ELF3 elf3 (Catalog #MBS420518) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ELF3/ERT/ ESX Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ELF3/ERT/ ESX can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 48 kDa band observed in NCI-H460 lysate (calculated MW of 41.5kDa according to NP_004424.2). In transfected HEK293 transiently expressing ELF3 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 2-4ug/ml. Researchers should empirically determine the suitability of the ELF3 elf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The ELF3 elf3 product has the following accession number(s) (GI #40255035) (NCBI Accession #NP_004424.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-ELF3/ERT/ ESX Antibody with the following immunoassay(s):
Western Blot (WB) (Staining (3ug/ml) of NCI-H460 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB) ELF3.

Western Blot (WB) (HEK293 overexpressing ELF3 (RC200631) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB) ELF3.

Immunogen: Peptide with sequence C-SGWKEEEVLQSRN, from the C Terminus of the protein sequence according to NP_004424.2.
Epitope: C Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ELF3 are readily searchable from our website. Different antibodies against the same target such as ELF3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Adenocarcinoma, Breast Neoplasms, Cell Transformation, Neoplastic, Fibrosis, Inflammation, Lung Diseases, Lung Neoplasms, Necrosis, Neoplasm Metastasis, Neoplasms, Experimental are some of the diseases may be linked to Goat anti-ELF3/ERT/ ESX Antibody. Bladder, Embryonic Tissue, Intestine, Kidney, Liver, Lung, Mammary Gland, Muscle, Prostate, Stomach tissues are correlated with this protein.
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